Leica M80 manual

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A good user manual

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- informations concerning technical data of Leica M80
- name of the manufacturer and a year of construction of the Leica M80 item
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- safety signs and mark certificates which confirm compatibility with appropriate standards

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Usually it results from the lack of time and certainty about functionalities of purchased items. Unfortunately, networking and start-up of Leica M80 alone are not enough. An instruction contains a number of clues concerning respective functionalities, safety rules, maintenance methods (what means should be used), eventual defects of Leica M80, and methods of problem resolution. Eventually, when one still can't find the answer to his problems, he will be directed to the Leica service. Lately animated manuals and instructional videos are quite popular among customers. These kinds of user manuals are effective; they assure that a customer will familiarize himself with the whole material, and won't skip complicated, technical information of Leica M80.

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Table of contents for the manual

  • Page 1

    LeicaM50 Leica M60 Leica M80 User Manual[...]

  • Page 2

    Leica M-series User Manual 2 General Instructions Safety concept Before using your microscope for the first time, please read the "Safety concept" br ochure included with y our instrument. It contains addi- tional information about handling and care . Use in clean rooms The Leica M series can be used in clean rooms without any problems .[...]

  • Page 3

    Leica M-series User Manual 3 Impor tant Safety Notes User Manual The individual modules of the Leica M stereo- microscopy series include an interactive CD-ROM with all relevant user manuals in 20other languages. Keep it in a safe place, and readily ac cessible to the user . User manuals and updates are also available for you to download and prin[...]

  • Page 4

    Leica M-series User Manual 4 Symbols U sed W arning of a danger • This symbol indicates especially impor- tant information that must be read and complied with. F ailure to comply can cause the f ollowing: ★ Hazards to personnel ★ F unctional disturbances or damaged instruments W arning of hazardous electrical voltage  This symbol indica[...]

  • Page 5

    Leica M-series User Manual 5 Safety Regulations Description ★ The individual modules fulfill the highest requirements for obser vation and docu- mentation of Leica stereomicr oscopes of the M series. Designated use ★ Refer to "Safety Conc ept" booklet Non-intended use ★ Refer to "Safety Conc ept" booklet Never use M serie[...]

  • Page 6

    Leica M-series User Manual 6 Safety Regulations (cont' d.) Repairs, service work ★ Refer to "Safety Conc ept" booklet ★ Only original Leica M icrosystems spare parts may be used. ★ Before opening the instruments, switch off the power and unplug the pow er cable. ★ T ouching the liv e circuit can cause injury. T ransport ★ [...]

  • Page 7

    Leica M-series User Manual 7 Safety Regulations (cont' d.) Light sources: saf ety regulations Prot ective measures of the manufacturer: ★ UV protection screen in front of the speci- men plane prevents the user from looking directly into the UV rays . ★ Dummy filter carriers in the free positions of the rapid filter changer prevent direct[...]

  • Page 8

    Leica M-series User Manual 8 Safety Regulations (cont' d.) Lamp housing ★ Never open the lamp housing while the lamp is switched on. Risk of explosion, UV exposure, blinding! ★ Before opening the lamp housing, allow it to cool off for at least 15 minutes. Danger of explosion! ★ Never cover the air duc t on the lamp hous- ing. Danger of [...]

  • Page 9

    Inhaltsverzeichnis General Instructions 2 Impor tant Safety Notes 3 Symbols Used 4 Safety Regulations 5 Safety Regulations (cont' d.) 6 Safety Regulations (cont' d.) 7 Safety Regulations (cont' d.) 8 The Leica M series Congra tulations! 12 The Modular Design: Everything is Relative 13 Maximum Compatibility 14 On We Go 15 Assembly Bas[...]

  • Page 10

    Photograph y & V ideo Photograph y & V ideo 44 Photo T ubes and C-mounts 45 Video/Phot otubes HD F & HD V 46 Objectives and Optical Acc essories The Differ ent T ypes of Objectives 48 Sy stem Illumination Leica LED3000 NVI™ (Near V ertical I llumination) 50 Leica LED5000 CXI (C oax Illumination) 51 Dimensional Drawings LeicaM50 53[...]

  • Page 11

    Leica M-series User Manual 11 The Lei c a M series[...]

  • Page 12

    Leica M-series User Manual 12 Congr atulations! The New Leica M Series The introduction of the Leica M50, M60 and M80 adds three new high- quality routine stereomicroscopes to the proven M-Series range from Leica M icrosystems . The optical brilliance and the wide range of acces- sories make them the ideal microscope for quality assurance and si[...]

  • Page 13

    Leica M-series User Manual 13 specific solutions. If you have a special request that cannot be met with standard parts, contact your Leica consultant. W e hav e a solution f or every problem. The Modular Design: E verything is Relative The Leica M series provides maximum flexibil- ity in choosing equipment, thanks primarily to the modular config[...]

  • Page 14

    Leica M-series User Manual 14 Leica engineers were careful to ensure that the new Leica M series—like its predecessors— remains compatible with existing series. T his means that objec tives, bases, tubes and so on can be reused. Objectives All objec tives of this new generation have the same ref erence f ocal length as the objectives of the MZ [...]

  • Page 15

    Leica M-series User Manual 15 On W e Go If your new Leica microscope has already been assembled and commissioned by your Leica consultant, click here to skip through the instal- lation instructions and go directly to the Quick Start Guide on Page 29. If, on the other hand, you are assembling the microscope yourself , continue with the " Assem-[...]

  • Page 16

    Leica M-series User Manual 16 Assembly[...]

  • Page 17

    Leica M-series User Manual 17 The first step is to c onnect the focusing column of the M series to the corresponding base . T ools used ★ Hex socket screw driver , 3mm Assembling the column adapter 1. Remove the three scr ews provided from the column. 2. Install the base on the column using the three screws . Tip: Place the column on the edge[...]

  • Page 18

    Leica M-series User Manual 18 T ools ★ Allen screwdriv er provided Assembly The microscope carrier is fastened t o the column using the screw pro vided. Alternativ e installation options Depending on the desired working distance , the microscope carrier can be installed in the stan- dard way or upside do wn (see figure below). Microscope Carrier[...]

  • Page 19

    Leica M-series User Manual 19 T ools used ★ None Assembling the optics carrier 1. Unscrew the clamping screw on the holder for the optics carrier . 2. Place the optics carrier into the holder and align it in such a way that the notch in the optics carrier and the bottom clamping screw are on t op of each other . 2. Tight en the clamping screw bel[...]

  • Page 20

    Leica M-series User Manual 20 T ube All intermediate tubes that fit between the optics carrier and the binocular tube are fitted in the same manner . T ools used ★ No tools requir ed. Prepara tion 1. Unscrew the positioning screw and remov e the protective co ver . Assembling the tube 2. Push the tube (for example , the inclined binocular tube)[...]

  • Page 21

    Leica M-series User Manual 21 Ey epieces T ools used ★ No tools requir ed. Magnification range Y ou can ex tend the overall mag nification range using available 10×, 16×, 25× and 40× wide - field eyepieces for persons w earing glasses. Prepara tion 1. If you want to use an optional gra ticule, insert it now (Page 43). 2. Remove the plastic[...]

  • Page 22

    Leica M-series User Manual 22 Objective T ools used ★ No tools requir ed. Prepara tion 1. Remove the protective cap on the optics carrier by turning it. Attaching the objective • Hold the objective firmly during assem- bly and disassembly so that it does not fall onto the stage plate. This applies par ticu- larly to the 2x planapochromatic [...]

  • Page 23

    Leica M-series User Manual 23 Leica LED5000 CXI (C oax Illumination) Required tools ★ None Installing the Leica LED5000 CXI 1. Connect the Leica LED5000 CXI to the focusing column via the CTL2 connec tion if the focusing column is equipped with inte - grated electronics . Alternativ e installation for manual columns 1. Connect the external power [...]

  • Page 24

    Leica M-series User Manual 24 3. Place the Leica LED5000 CXI on the optics carrier and tighten the positioning screw . 4. Push the tube (for example , the inclined binocular tube) into the dovetail ring and rotate it slightly in both directions until the positioning screw meshes with the guide groove. 5. While holding the tube only slightly , care-[...]

  • Page 25

    Leica M-series User Manual 25 Required tools ★ None  The Leica LED 3000 NVI™ can be used with any objec tives that have an outer diameter of 58mm.  The supported working distance is between 50mm and 150mm. Installing the Leica LED3000 NVI™ 1. Connect the Leica LED3000 NVI™ to the focusing column via the CTL2 connec tion if th[...]

  • Page 26

    Leica M-series User Manual 26 Leica LED3000 NVI™ (cont' d.) • Ensure that the locking screw of the illuminator and the locking screw of the optics carrier are on a str aight line, as otherwise image shading may occur .[...]

  • Page 27

    Leica M-series User Manual 27 Q uick Star t Guide[...]

  • Page 28

    Leica M-series User Manual 28 The Reliable Route t o Success Y our Leica stereomicrosc ope has been delivered in complet ely assembled condition by your Leica partner, and naturally you want to get right to work. This manual will familiarize you with the finer details of your micro- scope. The following pages contain impor tant, practical informat[...]

  • Page 29

    Leica M-series User Manual 29 Overview of an M Series M icroscope 1 Eyepieces for spectacle wearers with dioptric correction and eyecups 2 Fastening screws for the eyepieces 3 Ergo tube 4 Optics carrier 5 Magnification changer (zoom) 6 Interchangeable objective 7 Incident-light base 8 Column 9 Cable duct with cover 10 Coarse/fine focusing 5 10 2 [...]

  • Page 30

    Leica M-series User Manual 30 The C orrect Interpupillar y Distance The interpupillary distance is correctly set if you see a single circular image field when look ing at a specimen. If y ou are still a no vice micr oscope user , you may need a shor t time to become accustomed to this. Not to worry—after a little while, it will become automatic [...]

  • Page 31

    Leica M-series User Manual 31 If you wear glasses: 1. Hold the eyepiece firmly and rotate the eyecups counterclockwise towar ds the rear , as other wise the viewing distance is too great. 2. If an eyepiece is equipped with the inte - grated dioptric correction, turn the value to the "0" mark. By the way , one benefit of viewing with eye[...]

  • Page 32

    Leica M-series User Manual 32 F ocusing F ocusing raises or lowers the stereomicroscope using the focusing drive. The specimen detail is brought into sharp focus as soon as it is in the focal point of the objective. ★ The focusing drive can be operated either left- or right-handed. • The coarse/fine adjustment carries a load of up to 15 kg [...]

  • Page 33

    Leica M-series User Manual 33 Adjusting the Resistanc e of the Focus Driv e Adjusting the resistance Is the focus movement too loose or too tight? Does the outfit tend to slide downwar ds? The resistance can be adjusted individually depend- ing on the equipment weight and personal prefer ences as follows: 1. Grip the outer driv e knobs with both h[...]

  • Page 34

    Leica M-series User Manual 34 Changing the Magnification (Zoom) All M series microscopes have an integrated zoom. The name indicates the zoom range covered: ★ LeicaM50 = 5 increments ★ Leica M60 = 6:1 zoom ★ Leica M80 = 8:1 zoom The rotary knob f or the z oom can be used either left or right-handed. Zooming 1. Look int o the eyepieces. 2.[...]

  • Page 35

    Leica M-series User Manual 35 The zoom butt on can optionally be operated either with or without ratchet steps. Continu- ous z oom is possible when the ratchet steps are disabled, which many users find convenient. On the other hand, when the ratchet steps are enabled, photographs , measurement results etc. can be repr oduced more accura tely . Ena[...]

  • Page 36

    Leica M-series User Manual 36 E y epiec es & T ubes[...]

  • Page 37

    Leica M-series User Manual 37 Magnification F actor of the Ey epieces An eyepiece not only makes it possible to look passively into the micro- scope, but also has a critical effect on the maximum magnification. The magnification factor is between 10x and 40x. The following e yepieces are a vailable for the M series: Magnification Dioptric corr[...]

  • Page 38

    Leica M-series User Manual 38 Health Notes Pot ential sources of infection • Direct contact with eyepieces is a potential transmission method for bacterial and viral infections of the ey e. The risk can be kept to a minimum by using individual eyepieces or detachable eyecups. Eyecups can be ordered separat ely. Please contact your Leica partne[...]

  • Page 39

    Leica M-series User Manual 39 All Leica eyepiec es are also available with built- in dioptric correction, allowing the microscope to be used without glasses even by those with vision problems . The correction comprises ±5 diopter settings. Using the Dioptric Corr ec tion 1. S et the dioptric correction of both eyepieces to the mid position ("[...]

  • Page 40

    Leica M-series User Manual 40 Adjusting 1. S et the dioptric correction for both eyepieces to "0" . 2. S elect the lowest magnification and focus on a flat specimen. 3. S elect the highest magnification and read- just the sharpness. 4. S elect the lowest magnification again, but do not look into the eyepiec es. 6. Rotate the eyepieces[...]

  • Page 41

    Leica M-series User Manual 41 Graticules 2. Clamp the graticule on the insert, applying moderate pressure. Ensure tha t the grati- cule fits tightly . 3. S crew the insert and graticule firmly in to place and replace the ey epiece in the tube. 4. Y ou can now align the graticule by rotating the eyepiece in the tube and then tighten- ing it using [...]

  • Page 42

    Leica M-series User Manual 42 Ergo T ube™ 10° to 50° The Ergo T ube™ with continuously adjustable viewing angle from 10° to 50° allows for opti- mum sitting posture. Users who work with the same stereomicrosc ope can adjust the viewing height to their height with a simple hand move- ment. If the head posture is changed, the view- ing height[...]

  • Page 43

    Leica M-series User Manual 43 Photogr aph y & V ideo[...]

  • Page 44

    Leica M-series User Manual 44 F or most micr oscope users, digital documen- tation has become an invaluable par t of their work. Research results can be presented in an attractive manner ; measurements on the digi- tal image provide clarity and, in conjunction with the motorized IsoPro™ cross-stage, even images of large specimens can be captured [...]

  • Page 45

    Leica M-series User Manual 45 Photo T ubes and C-mounts Application All Leica DFC cameras are equipped with a standardized C-mount inter face. In turn, the C-mount adapter for the respective trinocular tube is connected t o this interface. This adapter creates a solid mechanical connection between the microscope and camera and ensures opti- mum ren[...]

  • Page 46

    Leica M-series User Manual 46 ★ Three-dimensional observation with 100% light in the binocular tube. The HD F features a fixed separa tion ratio of 50% in the binocular tube and 50% in the photo beam path. This allows the user to conduct three- dimensional obser vations of the samples in the binocular tube, manipulate and edit them and simult[...]

  • Page 47

    Leica M-series User Manual 47 Objec tiv es and Optic al A cc essories[...]

  • Page 48

    Leica M-series User Manual 48 The Differ ent T ypes of Objectives T o meet the various requirements regarding imaging properties, there is a choice of high- quality interchangeable planachromatic and planapochromatic objectives and also lower- priced inter changeable achromatic objectives. ★ Achroma tic objectives are par ticularly suited for sp[...]

  • Page 49

    Leica M-series User Manual 49 S y st em Illumina tion[...]

  • Page 50

    Leica M-series User Manual 50  The intensity of the illuminat or can be adjusted in 10 increments .  The Leica LED3000 NVI™ can also be controlled via the L eica Application Suite (LAS) or the Leica SmartT ouch.  The suppor ted working distance is between 50mm and 150mm. Use • The light of the Leica LED3000 NVI™ can be very [...]

  • Page 51

    Leica M-series User Manual 51  The intensity of the illuminat or can be adjusted in 10 increments .  The Leica LED3000 NVI™ can also be controlled via the L eica Application Suite (LAS) or the Leica SmartT ouch.  Using the L eica LED5000 CXI results in an increased magnification lev el of 1.5×.  Depending on the components used , di[...]

  • Page 52

    Leica M-series User Manual 52 Dimensional D ra wings[...]

  • Page 53

    Leica M-series User Manual 53 LeicaM50 LeicaM50 with small incident-light base, tr ansmitted-light base, Leica L2 illumination and 45° binocular tube 205 205 90 105 277 390 220 277 390 54 54 66 87 436 134 300 321 315 205 205 90 105 277 390 220 277 390 54 54 66 87 436 134 300 321 315 205 205 90 105 277 390 220 277 390 54 54 66 87 436 134 300 [...]

  • Page 54

    Leica M-series User Manual 54 LeicaM50 LeicaM50 with large incident-light base and Ergo T ube® 45° 278 54 301 278 119 116 301 322 24 436 514 500 255 278 54 301 278 119 116 301 322 24 436 514 500 255 278 54 301 278 119 116 301 322 24 436 514 500 255[...]

  • Page 55

    Leica M-series User Manual 55 LeicaM50 LeicaM50 with small swinging-arm stand and 45° binocular tube 54 170 500 173 220 709 260 260 345 54 54 170 500 173 220 709 260 260 345 54 54 170 500 173 220 709 260 260 345 54[...]

  • Page 56

    Leica M-series User Manual 56 Leica M60 Leica M60 with large incident light base and 45° binocular tube 278 301 278 119 116 301 288 324 24 330 54 54 66 255 278 301 278 119 116 301 288 324 24 330 54 54 66 255 278 301 278 119 116 301 288 324 24 330 54 54 66 255[...]

  • Page 57

    Leica M-series User Manual 57 Leica M80 Leica M80 with large incident-light base and 45° binocular tube 278 301 278 119 116 301 288 324 24 395 54 54 66 255 278 301 278 119 116 301 288 324 24 395 54 54 66 255 278 301 278 119 116 301 288 324 24 395 54 54 66 255[...]

  • Page 58

    Leica M-series User Manual 58 Leica M80 Leica M80 with transmitted-light base TL ST and binocular Ergo T ube® 340 430 85 154 154 106 21 500 290 420 606 356 340 430 85 154 154 106 21 500 290 420 606 356 340 430 85 154 154 106 21 500 290 420 606 356[...]

  • Page 59

    Leica M-series User Manual 59 Leica M80 Leica M80 with standard swinging-arm stand and 45° binocular tube 54 173 230 220 598 345 470 794 330 54 54 173 230 220 598 345 470 794 330 54 54 173 230 220 598 345 470 794 330 54[...]

  • Page 60

    Leica M-series User Manual 60 Leica M80 Leica M80 with Leica LED2500 and binocular Ergo T ube® 257 231 54 257 100 331 109 min. 290 max. 426 300 331 42 257 231 54 257 100 331 109 min. 290 max. 426 300 331 42 257 231 54 257 100 331 109 min. 290 max. 426 300 331 42[...]

  • Page 61

    Leica M-series User Manual 61 Leica M80 Leica M80 with universal stand, XL e xtension and binocular Ergo T ube® 600 400 54 292 369 min. 290 83.4 28.5 max. 420 631 600 600 400 54 292 369 min. 290 83.4 28.5 max. 420 631 600 600 400 54 292 369 min. 290 83.4 28.5 max. 420 631 600[...]

  • Page 62

    Leica M-series User Manual 62 Appendix[...]

  • Page 63

    Leica M-series User Manual 63 Calculating the T otal Magnification and F ield of Vie w Diameter Paramet er MO Magnifica tion of objective ME Magnification of eyepiece z Magnification changer position q T ube factor , e.g. 1.5× for coaxial incident light, 1.6× for 45° Ergo T ube™ r F actor 1.25x if the planachromatic and planapochromatic ob[...]

  • Page 64

    Leica M-series User Manual 64 Care , Maintenanc e, C ontac t P ersons W e hope you enjoy using your high-per for- mance microscope. Leica microscopes are renowned for their r obustness and long ser vice life. Observing the following care and clean- ing tips will ensure that even af ter years and decades, your Leica micr oscope will continue to work[...]

  • Page 65

    Leica M-series User Manual 65 Care , Maintenanc e, C ontac t P ersons (cont' d.) Protection from dirt Dust and dir t will affect the qualit y of your results. ★ Put a dust cover over the microscope when it will not be used for a long time. ★ Use dust caps to protect tube openings, tubes without eyepieces , and eyepieces. ★ Keep accessor[...]