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Leica RM2125 manual

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Table of contents for the manual

  • Page 1

    The Leica RM2125 Microtome Online T roubleshooting Guide[...]

  • Page 2

    Leica RM2125 T roubleshooting Guide T able of Contents Problem Page 1. Thick and thin sections 1-2 2. Compressed sections (sections are extremely compressed, wrinkled or jammed together) 3-4 3. Chatter 5-6 4. Scratches or splits in sections 7 5. No specimen advance (no section produced) 8 6. Drifting of handwheel 9 7. Specimen is picked up in the r[...]

  • Page 3

    Leica RM2125 T roubleshooting Guide Problem 1. Thick and thin sections a) Partial sections b) Shorter sections in ribbon c) Section thickness varies from one section to another d) Skipping sections e) V aried thickness in one individual section Cause(s) / Corrective Action I) The clearance angle is too small. a) Systematically try several clearance[...]

  • Page 4

    Leica RM2125 T roubleshooting Guide Problem 1. Thick and thin sections (continued) a) Partial sections b) Shorter sections in ribbon c) Section thickness varies from one section to another d) Skipping sections e) V aried thickness in one individual section Cause(s) / Corrective Action VI) Backlash in specimen advance system. l) T est for backlash b[...]

  • Page 5

    Leica RM2125 T roubleshooting Guide Problem 2. Compressed sections (sections are extremely compressed, wrinkled or jammed together) a) Compressed b) Wrinkled c) Jammed Cause(s) / Corrective Action I) Dull knife/blade. a) Use a different part of the cutting edge or use a new knife/blade. II) Blade/knife is gummed up with paraffin b) Clean knife with[...]

  • Page 6

    Leica RM2125 T roubleshooting Guide Problem 2. Compressed sections (sections are extremely compressed, wrinkled or jammed together) (continued) a) Compressed b) Wrinkled c) Jammed Cause(s) / Corrective Action VII) Knife blade is not parallel with pressure plate. (cont.) l) Adjust Pressure Plate with set screws at the bottom of the knife holder (tur[...]

  • Page 7

    Leica RM2125 T roubleshooting Guide Problem 3. Chatter a) V enetian Blind affect b) W ash board c) Undulations Cause(s) / Corrective Action I) Sectioning speed too high. a) Rotate the handwheel at a slower speed. II) Clearance angle too big. c) Clearance angle adjustment: Systematically decrease the clearance angle until the optimum adjustment is o[...]

  • Page 8

    Leica RM2125 T roubleshooting Guide Problem 3. Chatter (continued) a) V enetian Blind affect b) W ash board c) Undulations Cause(s) / Corrective Action – Decalcification – Paraffin is too old – Paraffin impurities IX) T abletop stability . k) Move the microtome or table to a more stable location, or replace the table if the table is flimsy . [...]

  • Page 9

    Leica RM2125 T roubleshooting Guide Problem 4. Scratches or splits in sections Cause(s) / Corrective Action I) Knife: – Defect in knife edge – Hard specimen in block – Paraffin on front or back of knife edge – Possible staple left in paraffin a) Move to a new area of the knife and observe if the scratches move with it. If so, replace the kn[...]

  • Page 10

    Leica RM2125 T roubleshooting Guide Problem 5. No specimen advance (no section produced) Cause(s) / Corrective Action I) Specimen reached the front feed limit. a) T urn the coarse feed wheel in the appropriate direction to move the specimen towards the rear limit. II) User may accidently be holding the coarse feed wheel while sectioning. III) The c[...]

  • Page 11

    Leica RM2125 T roubleshooting Guide Problem 6. Drifting of handwheel Cause(s) / Corrective Action I) Hand wheel is not balanced properly . Handwheel should remain at 12 o’clock when positioned at that location. No drifting should occur . a) Adjust the handwheel position and/or collar sleeve position. Service call is needed . 9 back to T able of C[...]

  • Page 12

    Leica RM2125 T roubleshooting Guide Problem 7. Specimen is picked up in the return stroke of the specimen arm Cause(s) / Corrective Action I) Static electricity charge may be built up on the knife holder or specimen head. a) Adjust the temperature or humidity level in the room. b) Use an ionizer to neutralize the static charge. c) Clean components [...]

  • Page 13

    Leica RM2125 T roubleshooting Guide Problem 8. Sections sticking to pressure plate Cause(s) / Corrective Action I) T oo much paraffin. a) Clean the knife with a swab moistened with Xylene. Stroke the swab in an upward motion away from the blade edge, not down on the blade edge. II) Dirty pressure plate. b) Clean the plate with a swab moistened with[...]

  • Page 14

    Leica RM2125 T roubleshooting Guide Problem 9. Unable to retract specimen (Note: This is only on the Leica RM2125 RT model) Cause(s) / Corrective Action I) Retraction mechanism on specimen advance system is defective. a) Schedule a service call. 12 back to T able of Contents[...]

  • Page 15

    Leica RM2125 T roubleshooting Guide Problem 10. Microtome makes "clunking noise" when sectioning Cause(s) / Corrective Action I) Linear bearings in cage are at their lower limits. a) Linear bearings need to be cleaned and possibly re-greased. II) Alignment of bearing cage is incorrect. b) Schedule a service call. 13 back to T able of Cont[...]