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Olympus FV1000 manual

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Table of contents for the manual

  • Page 1

    Confocal Laser Scanning Biological Microscope FV1000 FLUOVIEW FLUOVIEW—Always Evolving[...]

  • Page 2

    1 FLUOVIEW– —From Olympus is Open FLUOVIEW—Mor e Advanced than Ever The Olympus FLUOVIEW FV1000 confocal laser scanning microscope delivers efficient and reliable performance together with the high resolution required for multi-dimensional observation of cell and tissue morphology, and precise molecular localization. The FV1000 incorporates t[...]

  • Page 3

    2 Imaging to Analysis ing up New W orlds From Imaging to Analysis FV1000 Advanced Deeper Imaging with High Resolution FV1000MPE[...]

  • Page 4

    Advanced FLUOVIEW Systems Enhance the Power of Y our Resear ch Superb Optical Systems Set the Standard for Accuracy and Sensitivity . T wo types of detectors deliver enhanced accuracy and sensitivity , and are pair ed with a new objective with low chr omatic aberration, to deliver even better precision for colocalization analysis. These optical adv[...]

  • Page 5

    4[...]

  • Page 6

    Laser combiner/Fiber Diode Laser Greater stability, longer service life and lower operating cost are achieved using diode lasers. Laser Feedback Control Scanner unit is equipped with laser power monitor for feedback control enhancing stable laser output. Laser Compatibility Diode laser : 405 nm, 440 nm, 473 nm, 559 nm, 635 nm Gas laser : Multi-line[...]

  • Page 7

    Technology / Hardware IX81 PMT PMT PMT Galvanometer scanning mirrors Galvanometer scanning mirrors UIS2 objectives Specimen Pupil projection lens SIM Scanner * Microscope icity . Optical System Motorized Microscopes Compatible with Olympus IX81 inverted microscope, BX61WI focusing nosepiece and fixed-stage upright microscope, and BX61 upright micro[...]

  • Page 8

    Spectral Based Detection Flexibility and High Sensitivity Spectral detection using gratings for 2 nm wavelength resolution and image acquisition matched to fluorescence wavelength peaks. User adjustable bandwidth of emission spectrum for acquiring bright images with minimal cross- talk. Precise Spectral Imaging The spectral detection unit uses a gr[...]

  • Page 9

    Technology / Hardware SIM (Simultaneous) Scanner Unit Combines the main scanner with a dedicated laser light stimulation scanner for investigating the trafficking of fluorescent- labeled molecules and marking of specific live cells. Simultaneous Laser Light Stimulation and Imaging Performs simultaneous laser light stimulation and imaging to acquire[...]

  • Page 10

    Low Chromatic Aberration Objective Best Reliability for Colocalization Analysis A new high NA oil-immersion objective minimizes chromatic aberration in the 405–650 nm region for enhanced imaging performance and image resolution at 405 nm. Delivers a high degree of correction for both lateral and axial chromatic aberration, for acquisition of 2D a[...]

  • Page 11

    Technology / Hardware Switchable between Confocal and TIRFM Imaging Switchable between confocal and TIRFM imaging for localization of proteins on the cytoplasmic membrane surface and acquisition of sectioning images within cells. Software Control of TIRF Illumination Built-in laser provides TIRF illumination. Software can be used to tune the angle [...]

  • Page 12

    User -Friendly Software to Support Y our Resear ch. Time Controller Precisely synchronizes different experimental protocols including FRAP, FLIP and FRET by acceptor photo- bleaching and time-lapse. Save and open settings for later use. Wide Choice of Scanning Modes Several available scanning modes including ROI, point and high-speed bidirectional [...]

  • Page 13

    Technology / Hardware Re-Use Function Open previously configured scanning conditions and apply them to new or subsequent experiments. Help Guide Comprehensive help guide describes the functions and usage for each command, and overall sequence of operations. Multi Stimulation Software Configure multiple stimulation points and conditions for laser li[...]

  • Page 14

    Br oad Application Support and Sophisticated Experiment Contr ol. Measur ement Light Stimulation Multi- Dimensional Time-Lapse FRET Colocalization 3D Mosaic Imaging Multi-Color Imaging 3D/4D V olume Rendering 13[...]

  • Page 15

    Application 15,000 20,000 30,000 25,000 Time (ms) 35,000 40,000 200 400 600 800 1,000 1,200 1,400 1,600 1,800 2,000 2,200 2,400 Intensity CH1 CH2 CH1 CH2 Measurement Diffusion measurement and molecular interaction analysis. Light Stimulation FRAP/FLIP/Photoactivation/Photoconversion/Uncaging. Multi-Dimensional Time-Lapse Long-term and multiple poin[...]

  • Page 16

    This optional software module enables data acquisition and analysis to investigate the molecular interaction and concentrations by calculating the diffusion coefficients of molecules within the cell. Diverse analysis methods (RICS/ccRICS, point FCS/point FCCS and FRAP) cover a wide range of molecular sizes and speeds. Dif fusion Measur ement Packag[...]

  • Page 17

    Application/ Molecular Interaction Analysis 0 µs 0 ms 0 ms 1 ms 2 ms 3 ms 4 ms n ms 10 µs 20 µs 30 µs 40 µs 50 µs n µs Molecule size Small Large Spatial Correlation Algorithm When the spatial correlation algorithm is applied between pixels, a higher correlation is obtained as the speed of movement of the molecule nears the scanning speed. Wh[...]

  • Page 18

    Laser Light Stimulation The SIM scanner system combines the main scanner with a laser light stimulation scanner. Control of the two independent beams enables simultaneous stimulation and imaging, to capture reactions during stimulation. Multi-stimulation software is used to continuously stimulate multiple points with laser light for simultaneous im[...]

  • Page 19

    Application/ Molecular Interaction Analysis Photoconversion The Kaede protein is a typical photoconvertible protein, which is a specialized fluorescent protein that changes color when exposed to light of a specific wavelength. When the Kaede protein is exposed to laser light, its fluorescence changes from green to red. This phenomenon can be used t[...]

  • Page 20

    Significantly Improved Long Time-Lapse Thr oughput Equipped with motorized XY stage for repeated image acquisition from multiple points scattered across a wide area. The system efficiently analyzes changes over time of cells in several different areas capturing, large amounts of data during a single experiment to increase the efficiency of experime[...]

  • Page 21

    Application/ Molecular Interaction Analysis 3D Mosaic Imaging Mosaic imaging is performed using a high-magnification objective to acquire continuous 3D (XYZ) images of adjacent fields of view using the motorized stage, utilizing proprietary software to assemble the images. The entire process from image acquisition to tiling can be fully automated. [...]

  • Page 22

    Expandability to Support Diverse Application. Application Standard Functions Optional Functions Molecular interaction and Intracellular diffusion measurement molecular concentration Calculation of diffusion coefficients for intracellular molecules, and analysis of analysis — molecular binding and changes in molecular density. Supports a wide rang[...]

  • Page 23

    Expandability 350 350 300 300 400 400 450 450 500 500 550 550 600 600 650 650 700 700 750 750 800 800 405 LD 440 LD 473 LD 6 35 LD 55 9 LD 458 M ulti Argon 515 M ulti Argon 543 G ree n HeNe 488 Argon mCherry 22[...]

  • Page 24

    Fluorescence Illumination Unit Stand with Mercury lamp house, motorized shutter, and fiber delivery system for conventional fluorescence observation. Light introduction via fiber optic port. Transmitted Light Detection Unit External transmitted light photomultiplier detector and 100 W Halogen conventional illumination, integrated for both laser sca[...]

  • Page 25

    Expandability B C Fluorescence illumination unit *Optional unit Transmitted light detection unit IX81 IX81-ZDC Inverted motorized microscope BX61WI BX61 Upright motorized microscope LD635 laser LD559 laser HeNeG laser AOTF Laser combiner (Single-fiber type) AOTF Laser combiner (Dual-fiber type) 635 nm 543 nm Select either laser Multi Ar laser LD473[...]

  • Page 26

    Objectives NA W.D. (mm) DIC prism Revolving nosepiece MPLN5X 0.10 20.00 – WI-SSNP, WI-SRE3 UMPLFLN10XW 0.30 3.50 WI-DIC10HR WI-SSNP, WI-SRE3 UMPLFLN20XW 0.50 3.50 WI-DIC20HR WI-SSNP, WI-SRE3 LUMPLFLN40XW 0.80 3.30 WI-DIC40HR WI-SSNP, WI-SRE3 LUMPLFLN60XW 1.00 2.00 WI-DIC60HR WI-SSNP, WI-SRE3 LUMFLN60XW 1.10 1.5 WI-DIC60HR WI-SSNP, WI-SRE3 XLUMPLF[...]

  • Page 27

    Expandability Dimensions (mm) Weight (kg) Power consumption Microscope with scan unit BX61/BX61WI 320 (W) x 580 (D) x 565 (H) 41 — IX81 350 (W) x 750 (D) x 640 (H) 51 Fluorescence illumination unit Lamp 180 (W) x 320 (D) x 235 (H) 6.7 Power supply 90 (W) x 270 (D) x 180 (H) 3.0 AC 100-240 V 50/60 Hz 1.6 A Transmitted light detection unit 170 (W) [...]

  • Page 28

    FLUOVIEW website www .olympusfluoview .com • OLYMPUS CORPOARATION is ISO9001/ISO14001 certified. • Illumination devices for microscope have suggested lifetimes. Periodic inspections are required. Please visit our web site for details. • Windows is a registered trademark of Microsoft Corporation in the United States and other countries. All ot[...]