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Leica MZ16 F manual

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Table of contents for the manual

  • Page 1

    Leica MZ16 F User manual[...]

  • Page 2

    Leica MZ16 F – User manual 2 2 Leica MZ16 F – User manual Dear User , W e thank you for your faith in our product, and hope it brings you both enjoyment and success. With the new fully apochromatic Leica MZ16 F from Leica Microsystems, you have purchased the best-performing, manual fluorescence stereomi- croscope on the market. The Leica MZ16 F[...]

  • Page 3

    3 Leica MZ16 F – User manual Operating controls 1 Zoom magnification changer 2 Coarse/fine focusing drive 3 Double iris diaphragm 4 Rapid filter changer for four selectable filter sets 5 Filter holder for an individually selectable filter 6 Switch for activating/deactivating the UV shutter 7 T rinocular video/phototube 8 Adjustable tubes for adju[...]

  • Page 4

    Page 4. Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .18 4.1 Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18 4.1.1 Fluorescence technology . . . . . . . . . . .18 4.1.2 Functional principle . . . . . . . . . . . . . . . .18 5. Functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .18 5.1 S[...]

  • Page 5

    Leica MZ16 F – User manual 5 Leica MZ16 F – Safety concept 5 1. Safety concept 1.1 The user manual Included with your Leica MZ16 F fluorescence stereomicroscope is an interactive CD-ROM with all pertinent user instructions in German, English, French, Spanish, Italian, Portuguese, Dutch, Dan- ish, Swedish, Finnish and Greek. Keep it in a safe pl[...]

  • Page 6

    6 Leica MZ16 F – Safety concept 1.1.2 Symbols used Warning of a danger This symbol indicates especially important infor- mation that, if not observed, – can cause hazards to personnel, – can lead to functional disturbances and dam- aged instruments. Warning of hazardous electrical voltage This symbol indicates especially important infor- mati[...]

  • Page 7

    Leica MZ16 F – User manual 7 Leica MZ16 F – Safety concept Permitted uses With the Leica MZ16 F fluorescence stereomicro- scope, entire fluorescent specimens can be examined three-dimensionally and unprepared, manipulated, sorted and documented. The sepa- rate beam path for the fluorescence illumination in the Leica MZ16 F as well as the very b[...]

  • Page 8

    8 Leica MZ16 F – Safety concept Repairs and servicing – Only Leica Microsystems-trained service tech- nicians, or technical specialists assigned by the person in charge of the instrument, are permitted to carry out repairs. – Only original Leica Microsystems spare parts may be used. – Disconnect the power cable before opening voltage-carryi[...]

  • Page 9

    Leica MZ16 F – Safety concept 9 1.3 Light source: safety instructions Precautions by the manufacturer – UV protection screen in front of the specimen plane prevents the user from looking directly into the UV rays. – Simple filter holders (that have only two open- ings for the observation beam paths) in the avail- able positions of the rapid f[...]

  • Page 10

    10 Leica MZ16 F – Overview of the instrument 2. Overview of the instrument 1 Stand (transmitted or incident light stand with corresponding illumination). Pictured here is the HL RC™ transmitted-light base for best relief contrast. 2 Focusing drive: (pictured) manual coarse/fine drive with column (see User Manual M1-105-0) or motorized focus sys[...]

  • Page 11

    Leica MZ16 F – Overview of the instrument 11 7 Filter sets: the scope of delivery includes three simple filter holders (which have only two openings for the observation beam paths) for empty filter positions and transmitted or inci- dent light observation without fluorescence. Additionally , various filter sets of your choice for fluorescence (ex[...]

  • Page 12

    12 Leica MZ16 F – Assembly 3. Assembly Basic components such as the stand, column, microscope carrier , binocular tubes and optical accessories are, thanks to their modular design, identical for all Leica M series stereomicro- scopes. Therefore, for assembly instructions, please refer to the User Manual for Leica M stereomicroscopes, M2-105-0. Th[...]

  • Page 13

    Leica MZ16 F – Assembly 13 10 445 631 10 445 367 HL 10 445 363 10 445 387 10 445 822 10 446 229 Filter Sets 10 447 220 10 447 221 10 447 217 10 447 222 10 447 218 10 447 223 10 447 219 10 447 224 10 447 225 10 447 226 10 447 227 10 447 215 10 447 216 10 446 271 10 447 106 / 10 447 185 Motor focus 10 446 176 / 10 447 041 MZ16 FA 10 447 063 10 447 [...]

  • Page 14

    14 Leica MZ16 F – Assembly 3.2 Assembly of stereomicroscope with stand Do not yet connect the peripherals to the power supply! Focusing drive ➔ stand base 왘 Fasten the manual or motorized focusing drive (4.2) to the column according to User Manual M2-105-0 (manual) or M2-267-1 (motorized) on the base (4.1). T ool: Allen key . 왘 For motorize[...]

  • Page 15

    Leica MZ16 F – Assembly 15 3.3 Additional components 왘 Fasten the other components and other individ- ual accessories according to User Manual M2-105-0: – Insert the binocular tube (2.5) or the video/ phototube into the dovetail ring on the FLUOIII ® filter system and fasten it using a clamping screw . – Insert the eyepieces (4.6) in the t[...]

  • Page 16

    16 Leica MZ16 F – User manual Leica MZ16 F – Assembly 3.5 UV protection screen ➔ microscope carrier 왘 Fasten the UV protection screen (5.1) using a hexagon-head screw (5.3) to the left or right side of the microscope carrier . T ool: Allen key . Always position the UV protection screen so that the operator can never look directly at the lig[...]

  • Page 17

    Leica MZ16 F – User manual 17 Leica MZ16 F – Assembly 1 2 3.6.2 Simple filter holders Three simple filter holders are included in the scope of delivery of the Leica MZ16 F . These filter holders have two empty openings for the observa- tion beam paths. The third opening, to the illumi- nation beam path, is closed. Use these filter holders – i[...]

  • Page 18

    18 Leica MZ16 F – Operation 4. Operation 4.1 Description 4.1.1 Fluorescence technology Fluorescent substances light up (fluoresce) when irradiated with short-wave excitation light. Fluo- rescence technology makes use of this property by coloring or marking certain object structures and details that do not have their own fluores- cence with a fluo[...]

  • Page 19

    Leica MZ16 F – Functions 19 5.1.1 Observation without fluorescence The FLUOIII ® filter system includes a UV shutter that can be used to close the illumination beam path. This feature protects the mercury lamp, which should not be switched on and off too fre- quently . If, for instance, you need to interrupt your work, you do not have to switch [...]

  • Page 20

    20 Leica MZ16 F – Functions 5.3 Rapid filter changer The rapid filter changer must be equipped with four filter holders at all times. If you are using fewer than four filter hold- ers with fluorescence filters, always insert the included simple filter holders into the available positions. Otherwise there is a risk of danger to the eyes from direc[...]

  • Page 21

    Leica MZ16 F – Work flow 21 6. W ork flow 왘 Switch on the supply unit (page 15). W ait for 2 to 3 minutes. 왘 Close the illumination beam path using the switch (10.1). 왘 Correctly set the binocular tube, eyepieces and working distance in accordance with the User Manual M2-105-0. 왘 Observe the specimen at the lowest magnifica- tion level fi[...]

  • Page 22

    22 Leica MZ16 F – Appendix 7. Appendix 7.1 Filter sets Filter sets Excitation filter Barrier filter Designation GFP 425/60 nm (395–455 nm) 480 LP GFP1 GFP Plus 480/40 nm (460–500 nm) 510 LP GFP2 GFP Plants 470/40 nm (450–490 nm) 525/50 nm (500–550 nm) GFP3 UV 360/40 nm (340–380 nm) 420 LP UV Violet 425/40 nm (405–445 nm) 460 LP V Blue[...]

  • Page 23

    Leica MZ16 F – Appendix 23 7.2 T echnical data Optics carrier Leica MZ16 F Microscope type stereo fluorescence microscope with apochromatic optics system, patented T ripleBeam ® third beam path, patented FLUOIII ® fluorescence filter system, and manual functions 100% apochromatic CMO (Common Main Objective) design with two parallel optics syste[...]

  • Page 24

    24 Leica MZ16 F – Appendix Leica MZ16 F Optical Data Zoom 16:1 manual, apochromatic Selectable zoom positions Click-stops at 0.71 | 1 | 1.6 | 2 | 2.5 | 3.2 | 4 | 5 | 6.3 | 8 | 10 | 11.5 for repetitive tasks Performance with standard optics (1 × objective/10 × eyepieces) – Zoom range 7.1 × – 115 × – Resolution 420 Lp/mm – Numerical ape[...]

  • Page 25

    Leica MZ16 F – Appendix 25 Stands, illuminators Leica MZ16 F T ransmitted light stand HL RC™ high-performance base with innovative contrast meth- ods, stands for bright field and bright/dark field Incident light stand With stage plate, black/white Stand for large objects Swinging arm stand, stage clamp stand, universal stand Motor focus With 30[...]

  • Page 26

    26 Leica MZ16 F – Appendix Leica MZ16 F T echnical Data Weights – Leica MZ16 F optics carrier – Lamp housing 2.5 kg – Filter set 20 g – Planapochromatic objective 1 × 0.74 kg – Focusing drive, coarse/fine, with column 1.15 kg – Microscope carrier 0.495 kg ebq 100 supply unit For indoor use only . – Supply voltage 90–250 V~ – Fr[...]

  • Page 27

    Leica MZ16 F – User manual 27 Leica MZ16 F – Appendix 27 7.3 Optical data Field of view diameter (mm) Eyepieces Working distances in mm Objectives 0.71 28.4 8.4 14.2 16.9 17.9 13.4 22.7 10.5 45.4 5.3 56.8 4.2 0.8 32 7.5 16 15 20.5 11.7 25.6 9.4 51.2 4.7 64 3.8 1 40 6 20 12 25.6 9.4 32 7.5 64 3.8 80 3 1.25 50 4.8 25 9.6 32 7.5 40 6 80 3 100 2.4 [...]

  • Page 28

    7.4 Dimensions 28 Leica MZ16 F – Appendix Dimensions in mm[...]

  • Page 29

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  • Page 30

    Leica Microsystems – the brand for outstanding products Leica Microsystems – an international company with a strong network of customer services Australia: Gladesville, NSW T el . + 1 800 625 286 Fax +61 2 9817 8358 Austria: Vienna T el . +43 1 486 80 50 0 Fax +43 1 486 80 50 30 Canada: Richmond Hill/Ontario Tel . + 1 905 762 20 00 Fax +1 905 7[...]